When you open Photoshop, you are prompted to choose between RBG and CMYK. At first glance, these may seem like options you can ignore- arbitrary and without impact. However, they are not. Each represents a different approach to color in terms of how it is created and displayed. Journals are meticulous about figures. For example, the Biophysical Journal requires all submissions to be in the RBG format. The journal previously required images to be submitted in RBG, converted to CMYK, and then converted again to RBG for online publications. However, this resulted in a loss of image quality, prompting the journal to change its submission requirements.